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KMID : 0368420090520030251
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Journal of Plant Biology
2009 Volume.52 No. 3 p.251 ~ p.258
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Assessment of Gene Flow from Genetically Modified Anthracnose-Resistant Chili Pepper (Capsicum annuum L.) to a Conventional Crop
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Kim Chang-Gi
Kim Dae-In
Kim Hyo-Jeong
Park Ji-Eun
Lee Bum-Kyu
Park Kee-Woong
Jeong Soon-Chun
Choi Kyung-Hwa
An Joo-Hee
Cho Kang-Hyun
Kim Young-Soon
Kim Hwan-Mook
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Abstract
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We conducted a 2-year field assessment of the gene flow from genetically modified (GM) chili pepper (Capsicum annuum L.), containing the PepEST (pepper esterase) gene, to a non-GM control line “WT512” and two commercial hybrid cultivars, “Manidda” and “Cheongpung Myeongwol (CM).” After seeds were collected from the pollen-recipient non-GM plants, hybrids between them and the GM peppers were screened by a hygromycin assay. PCR with the targeting hpt gene was performed to confirm the presence of transgenes in hygromycin-resistant seedlings. Out of 7,071 “WT512” seeds and 6,854 “Manidda” seeds collected in 2006, eight and 12 hybrids, respectively, were detected. In 2007, 33 hybrids from 3,456 “WT512” seeds and 50 hybrids from 3,457 “CM” seeds were found. The highest frequency of gene flow, 6.19%, was observed in that 2007 trial. These results suggest that a limited isolation distance would be sufficient to prevent gene flow from GM to conventionally bred chili peppers.
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KEYWORD
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Capsicum annuum, Chili pepper, Gene flow, Genetically modified (GM) crop
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